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Encompassing Amino Acids: 1-663(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction was performed in a buffer (50 mM Tris, pH 7.4, 50mM NaCl, 10 mM CaCl2, and 2 mMDTT) containing PADI4 and 10 mMBAEE as a substrate for 25 min at 37°C.Amount of ammonia produced wasmeasured and used for specific activitycalculation.
Description: Human PADI-4, also known as Peptidyl arginine deiminase, type IV, (GenBank Accession No. NM_012387), 1-663(end), with C-terminal FLAG-tag, MW= 75 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 µg/ml FLAG peptide
Genbank: NM_012387
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: Q9UM07
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Kanno T, et al. (2000). J. Invest. Dermatol. 115 (5): 813-23.
2. Nachat R, et al. (2005). J. Invest. Dermatol. 124 (2): 384-93.
3. Wang Y, et al. (2004). Science 306 (5694): 279-83.
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