Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and HTS applications.
Background: Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, play an essential role in polypeptide processing during virus replication. PLPro, in addition to being crucial during replication via processing of the viral polyprotein, is proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV. This includes deubiquitination (the removal of ubiquitin) from host-cell proteins. The last enzymatic activity result in the antagonism of the host antiviral innate immune response. As a result, PLpro is an important potential target for antiviral drugs that may inhibit viral replication and weaken dysregulation of signalling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
Description: The Papain-like Protease Assay Kit: Deubiquitinase Activity is designed to measure Papain-like Protease deubiquitinating activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
Storage Stability: At least six months from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient 96-well format, with purified Papain-like Protease, ubiquitinated fluorogenic substrate, and PLPro assay buffer for 100 enzyme reactions. PLPro inhibitor GRL0617 is also included as a positive control.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Ewelina Weglarz-Tomczak, et al., 2020. https://doi.org/10.1101/2020.05.17.100768.
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