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Application: Great for studying enzyme kinetics and HTS applications.
Background: Coronaviruses (CoVs) primarily cause multiple respiratory and intestinal infections in humans and animals. Papain-Like Protease (PLPro), also known as PLP, plays an essential role in polypeptide processing during virus replication. PLProis also proposed to be a key enzyme in the sustained pathogenesis of SARS-CoV-2. PLP acts as a deubiquitinase that removes ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate antiviral immune responses. As a result, PLPro is an important potential target for antiviral drugs that may inhibit viral replication and simultaneously weaken dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells. PLPro inhibitors that can block viral replication are promising potential drug candidates that could be used to treat patients suffering with the COVID-19 coronavirus infection.
Description: The Papain-like Protease Assay Kit: Protease Activity is designed to measure PLPro activity for screening and profiling applications, in a homogeneous assay with no time-consuming washing steps.
Storage Stability: At least six months from date of receipt when stored as directed.
Supplied As: The kit comes in a convenient 96-well format, with purified Papain-like Protease, fluorogenic substrate, and PLPro assay buffer for 100 enzyme reactions. PLPro inhibitor GRL0617 is also included as a positive control.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Weglarz-Tomczak, E. et al., 2020. https://doi.org/10.1101/2020.05.17.100768.
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