Products from BPS Bioscience require a minimum order value above 400€Applications:
- Use as a negative control when testing PARG inhibitors in HeLa cells.
- Study the phenotype of PARG knockout.
- Introduce further CRISPR/Cas9-based genetic manipulations in order to understand the interplay between PARG and other partners and pathways.
Background: Poly (ADP-ribose) glycohydrolase (PARG) is a catabolic enzyme involved in the degradation of PARylated chains, releasing ADP-ribose and oligo (ADP-ribose) chains. PAR (poly-ADP ribosylation) homeostasis is regulated by the family of PAR polymerases (PARPs) and PARG in response to cellular stress conditions such as DNA damage response (DDR). PARG activity is linked to cellular responses in inflammation, ischemia, stroke, and cancer. PARG is overexpressed in breast cancer and associated with tumor growth and survival. Decrease in PARG activity can potentiate the effect of current cancer therapies, such as chemotherapy and radiation, making PARG inhibition with selective inhibitors a promising approach in cancer and immunotherapy.
Description: PARG Knockout HeLa Cell Line is a HeLa cell line in which PARG (Poly ADP-ribose glycohydrolase) has been genetically removed using CRISPR/Cas9 genome editing with a lentivirus encoding CRISPR/Cas9 gene and sgRNA (single guide RNA) targeting human PARG.
Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.
Storage Stability: Cells will arrive in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use a -80°C freezer for long term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.
Supplied As: Each vial contains ~1 x 10
6 cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)
Uniprot: Q86W56
Warnings: Avoid freeze/thaw cycles
Biosafety Level: BSL-2
References: Marques M.,
et al., 2019
Oncogene 38 (12): 2177-2191.
James D. I., et al., 2016
ACS Chem Biol 11 (11): 3179-3190.
Drown B. S.,
et al., 2018
Cell Chem Bio 25 (12): 1562-1570.
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