Products from BPS Bioscience require a minimum order value above 400€Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PARP1 Chemiluminescent Activity Assay Kit is designed to measurePARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependentaddition of poly (ADP-ribose) to histones. The key to the PARP1 ChemiluminescentActivity Assay is the biotinylated substrate. With this kit, only three simple steps are required forPARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme.Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate toproduce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PARP1 assay kit comes in a convenient 384-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 400 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41.Application Reference(s):
Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis (2013)
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