Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PARP10 has been shown to catalyze NAD-dependent mono-ribosylation.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PARP10 Chemiluminescent Assay Kit is designed to measure PARP10 activity for screening and profiling applications. The key to the PARP10 Chemiluminescent Assay Kit is the biotinylated substrate. With this kit, only three simple steps are required for PARP10 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP10 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PARP10 assay kit comes in a convenient 96-well format, with purified PARP10 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)