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Encompassing Amino Acids: 500-701(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Ribosylationreaction is conducted in wells coated with amixture of histones at room temperature for 1hour. Assay buffer: 50 mM Tris-HCl (pH 8.0),5 mM MgCl2 and 20 µM ZnCl2 .
Description: Human PARP12 (GenBank Accession No. NM_022750), a.a. 500-701(end) with N-terminal His-GST-tag, MW = 51 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 8 mM glutathione, 0.04% Tween-20, and 20% glycerol.
Genbank: NM_022750
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-GST tag
Uniprot: Q9H0J9
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Katoh,M. and Katoh,M. Int. J. Oncol. 23 (2), 541-547 (2003).
2. Hillier,L.W., et al. Nature 424 (6945), 157- 164 (2003).
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