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Encompassing Amino Acids: 1470-1801(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted usingBPS's PARP14 Assay Kit (Catalog #80568) at room temperature for 1 hour.
Description: Human PARP14 (GenBank Accession No. NM_017554), a.a. 1470-1801(end) with N-terminal His-GST-tag, MW = 66.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 188 mM imidazole, 0.04% Tween-20, 3 mM DTT, 20% glycerol.
Genbank: NM_017554
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q460N5
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Barbarulo, A., et al., Oncogene. 10 (1038), 448 (2012).
2. Cho, SH., et al., Proc Natl Acad Sci U S A. 108(38),15972-7 (2011).Product Specific Reference(s): 1. Wang, P., et al., Tetrahedron. 70(35), 5666-5673 (2014).
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