Products from BPS Bioscience require a minimum order value above 400€Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Contraindications: The PARP15-FL Chemiluminescent Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.
Description: The full length PARP15 (PARP15-FL) Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. PARP15 is known to catalyze the NAD-dependent ADP-ribosylation of histones. This Assay Kit takes advantage of the sensitive detection of biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP15-FL enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Illustration 1: PARP15-FL Chemiluminescent Assay Kit schematic
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Supplied As: The PARP15-FL assay kit comes in a convenient 96-well format, with purified full length PARP15 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Uniprot: Q460N3
Warnings: Avoid freeze/thaw cycles
Biosafety Level: Not applicable (BSL-1)
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