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Encompassing Amino Acids: 221-444(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted usingBPS's PARP15 Assay Kit (Catalog #80567) at room temperature for 30 minutes.
Description: Human poly (ADP-ribose) polymerase family, member 15 (PARP15) (GenBank Accession No. NM_152615, var.2), a.a. 221-444(end) with N-terminal GST-tag, MW = 52.5 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol.
Genbank: NM_152615, var2
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q460N3
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Andersson CD., et al. J. Med. Chem. 2012 Sep 13;55(17):7706-18.
2. Ricardo C. T. Aguiar., et al. J. Biochem.2005 Oct 280;(40):33756-65.
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