Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PARP3 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PARP3 Chemiluminescent Activity Assay kit is designed to measure PARP3 activity for screening and profiling applications. The key to the PARP3 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP3 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with the assay buffer that contains the PARP3 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PARP3 assay kit comes in a convenient 96-well format, with purified PARP3 enzyme, histone mixture, activated DNA, and PARP assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
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