Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PARP6 is known to catalyze the NAD-dependent addition of poly(ADP-ribose) to histones.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PARP6 Chemiluminescent Activity Assay kit is designed to measure PARP6 activity for screening and profiling applications. The key to the PARP6 chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP6 reactions. First, histone proteins are coated on a microplate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP6 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Kit comes in a convenient plate format, with purified PARP6 enzyme, histone mixture, activated DNA, and PARP assay buffer for 32 enzyme reactions*.
Note: The kit includes sufficient amounts of histones, substrate, buffer, DNA and detection reagent
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
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