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Application: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: PARP7, also known as TCDD-inducible poly(ADP-ribose) polymerase or TIPARP, catalyzes NAD-dependent ribosylation.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The PARP7 Chemiluminescent Activity Assay kit is designed to measure PARP7 activity for screening and profiling applications. The key to the PARP7 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP7 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP biotinylated substrate is incubated with an assay buffer that contains the PARP7 enzyme. Finally, the plates are treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Storage Stability: Up to 1 year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PARP7 assay kit comes in a convenient 96-well format, with purified PARP7 enzyme, histone mixture, and PARP assay buffer for 100 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
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