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Encompassing Amino Acids: 400-657 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: >40 pmol/min/µg Assay Conditions: Assay was done according to PARP7 assay kit protocol #80557 at room temperature for 1 hour..
Description: Human PARP7 (GenBank Accession No. NM_015508), a.a. 400-657 (end) with N-terminal FLAG-tag, MW = 31 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 3 mM DTT, 20% glycerol and 100 µg/mL FLAG peptide.
Genbank: NM_015508
Storage Stability: At least 6 months at -80°C. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal FLAG-tag
Uniprot: Q7Z3E1 Q0PNE2
Unit Definition: One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into the acid-insoluble form.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rouleau M., et al. J Cell Biochem. 2007 Feb 1;100(2):385-401.2. Augustin A., et al. J Cell Sci. 2003 Apr 15;116(Pt 8):1551-62.
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