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Applications: This kit is most useful for screening for inhibitors of PD-L1.
Description: Cell signaling through the PD-1 receptor upon binding the PD-L1 ligandattenuates immune responses and is exploited by both tumors and viruses. The PD-1[Biotinylated]:PD-L1 Inhibitor Colorimetric Screening Assay Kit is designed for screeninginhibitors of PD-1 receptor binding to PD-L1 ligand. This kit comes in a convenient 96-wellformat, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for100 binding reactions. The key to this kit is the high affinity of biotin-labeled PD-1 forstreptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First,PD-L1 is coated on a 96-well plate. Next, PD-1 is incubated with PD-L1 on the plate. Finally, theplate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate toproduce color, which can then be measured using a UV/Vis spectrophotometer microplatereader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled PD-1, purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Lin, D., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 3011-3016.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.
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