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Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Assay Conditions: BPS Cat. 72005protocol was followed. Briefly, 50 µl of 2µg/ml PD-L1 was coated onto plates. 20 µlof 0.5 µg/ml PD-1, Biotin-labeled wasallowed to bind at room temp. for 2 hours.Strep-HRP was added and allowed toincubate at room temp for 1 hr, followed byHPR chemiluminescent substrate additionand plate reading.
Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag ™ expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag™ technology. MW = 44.5 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Genbank: NM_005018
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.