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Applications: Useful for labeling PD-L1- expressing cells for flow cytometry, immunofluorescence, and live cell imaging. For FACS analysis on human cells expressing PD-1, we recommend preclearing by using a non-specific antibody at 10 ug/ml to remove any non-specific binding, followed by PE labeled PD-1 fusion protein at 20 ug/ml.
Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279 and HPD-L, GenBank Accession No. NM_005018, a.a 25-167, fused at the Cterminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.
Format: Aqueous buffer solution
Formulation: 8 mM Na phosphate pH 7.4,110 mM NaCl, 2.2 mM KCl and 20% glycerol
Genbank: NM_005018
Storage Stability: At least 6 months at -80°C.
Tags: Fc Fusion (Human IgG1)
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protect from light. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shinohara, T., et al., Genomics. 1994 Oct; 23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005 Aug; 6(5):430-7.
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