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Applications: Useful for studying protein binding and screening small molecular inhibitors
Description: Human secreted programmedcell death 1 (PD-1), also known as PDCD1,SLEB2, CD279 and HPD-L, GenBankAccession No. NM_005018, a.a. 25-167,with C-terminal FLAG-Avi-His-tags, MW=20kDa, expressed in a HEK293 cellexpression system. The protein isenzymatically biotinylated using AviTag™technology. This protein runs at a higherM.W. by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_005018
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-Avi-His-tags
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shinohara, T., et al., Genomics. 1994Oct;23(3):704-6.
2. James, E.S., et al., Genes Immun. 2005Aug;6(5):430-7.
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