PD-1/IL-2 Luciferase Reporter Jurkat Cell Line

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Applications: Screen for inhibitors of PD-1 or PD-L1 signaling in a cellular model.Characterize the biological activity of PD-1 and interaction with its ligands.

Background: PD-L1 and PD-L2 binding to PD-1, a receptor expressed on T cells, negatively regulates immune responses. PD-1 ligands PD-L1 and PD-L2 are found on the surface of most cancer cells, and their interaction with receptor PD-1 inhibits T cell activity and allows cancer cells to escape immune surveillance. This pathway is also involved in regulating autoimmune responses. Therefore, these proteins (termed immune checkpoints) are promising therapeutic targets for many types of cancer as well as multiple sclerosis, arthritis, lupus, and type I diabetes. Checkpoint inhibitors have remarkable efficacy in a wide range of cancer types and have revolutionized cancer treatment. PD-1 inhibitors nivolumab, pembrolizumab, cemiplimab and PD-L1 inhibitors atezolizumab, avelumab, and durvalumab are all FDA-approved drugs for immuno-therapy.

Description: PD-1/IL-2 Luciferase Reporter Jurkat Cell Line is a Jurkat T cell line constitutively expressing human PD-1 (Programmed Cell Death 1, also known as PDCD1, SLEB2, CD279, GenBank Accession #NM_005018), and conditionally expressing firefly luciferase under the control of a human interleukin-2 (IL-2) promoter. This cell line has been validated by flow cytometry for expression of PD-1, and in co-culture assays that assess the PD-L1/PD-1 interaction on Jurkat T cell activation.

Figure 1: Illustration of the mechanism of action of PD-1/IL-2 Luciferase Reporter Jurkat Cell Line in a co-culture assay.
The TCR activator presented at the surface of PD-L1/TCR Activator CHO cells stimulates TCR (T cell receptor) in Jurkat T cells, whereas overexpression of PD-L1 on the CHO cell line engages Jurkat PD-1 resulting in blocking TCR signaling and preventing activation of the IL-2 promoter. Addition of a neutralizing anti-PD-1 or anti-PD-L1 antibody to the co-culture prevents formation of the PD-L1/PD-1 complex, and results in TCR activation and increased IL-2 promoter activity, which translates into increased luciferase reporter signal.

Host Cell Line: Jurkat (clone E6-1), human T lymphoblast, suspension

Mycoplasma Testing: The cell line has been screened to confirm the absence of Mycoplasma species.

Storage Stability: Cells are shipped in dry ice and should immediately be thawed or stored in liquid nitrogen upon receipt. Do not use an -80°C freezer for long-term storage. Contact technical support at support@bpsbioscience.com if the cells are not frozen in dry ice upon arrival.

Supplied As: Each vial contains ~1 x 10⁶ cells in 1 ml of Cell Freezing Medium (BPS Bioscience #79796)

Uniprot: PD-1: Q15116; IL-2: P60568

Warnings: Avoid freeze/thaw cycles

Biosafety Level: BSL-1

References: Sasca D, et al. 2019 Blood 133: 2305-2319

• SKU: BPS82117
• Manufacturer: BPS Bioscience
• Manufacturer SKU: 82117
• Package Unit: 2 vials
• Quantity Unit: PAK
• Host: Human