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Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Description: Human secreted Programmed Cell Death 1(PD-1)-Fc fusion protein, also known asCD279, PDCD1, and SLEB2, GenBankAccession No. NM_014143, a.a. 25-167with an P130A mutation, fused at the Cterminuswith the Fc portion of human IgG1,and C-terminal AviTag™, expressed in aHEK293 cell expression system andenzymatically biotin-labeled using AviTag™technology. MW = 44.5 kDa. This proteinruns at a higher MW by SDS-PAGE due toglycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4,110 mM NaCl, 2.2 mM KCl, and 20%glycerol
Genbank: NM_014143
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Avi-Tag™
Uniprot: Q15116
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al., J. Exp. Med. 2000 Oct 2;192(7):1027-34.2. Dong, H., et al., Nat Med. 1999. Dec;5(12):1365-9.
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