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Description: The PD-1:PD-L1 TR-FRET Assay is designed to measure the inhibition of PD-1 binding to PD-L1 in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing europium-labeled (Eu) PD-1, dye-labeled acceptor, biotin-labeled PD-L1, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.
Storage Stability: At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Molnar, E., et al. Proc Natl Acad Sci USA. 2008; 105: 10483-10488.2. Keir, M.E., et al. Annu. Rev. Immunol. 2008; 26: 677-704.