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Applications: This kit is most useful for screening for inhibitors of PD1 binding to PD-L2.
Description: Cell signaling through the PD-1 receptor upon binding the PD-L2 ligandattenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL2[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors ofPD-L2 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L2 for streptavidin-HRP.Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated ona 96-well plate. Next, PD-L2 is incubated with PD-1 on the plate. Finally, the plate is treated withstreptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, whichcan then be measured using a UV/Vis spectrophotometer microplate reader.
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled PD-L2, purified PD-1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Molnar, E., et al. Proc Natl Acad Sci U.S.A. 2008, 105: 10483-10488.
2. Keir, M.E., et al. Annu. Rev. Immunol. 2008, 26: 677-704.
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