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Applications: This kit is useful for screening for inhibitors of PD-L1 binding to B7-1.
Background: There have been a number of studies defining a role for PD-L1 in theregulation of immune tolerance and cancer immunity through binding to T cell-expressed PD-1.Interestingly, PD-L1 has also been shown to interact with B7-1, also known as CD80. Like PD-1:PD-L1 interaction, B7-1:PD-L1 signaling also leads to inhibition of T cell activation,proliferation, and cytokine production, suggesting that this pathway may also serve as a targetin cancer immunotherapy and inflammatory diseases.
Description: The PD-L1:B7-1[Biotinylated] Inhibitor Screening Assay Kit is designed forscreening and profiling inhibitors of the PD-L1:B7-1 interaction. The key to this kit is the high sensitivity of detection ofbiotin-labeled B7-1 by streptavidin-HRP. Only a few simple steps on a microtiter plate arerequired for the assay. First, PD-L1 is coated on a 96-well plate. Next, B7-1-biotin is incubatedwith PD-L1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by additionof an HRP substrate to produce chemiluminescence, which can then be measured using achemiluminescence reader.
Storage Stability: Stable for one year from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled B7-1 (CD80), purified PD-L1, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Butte, M.J., et al., Immunity. 2007; 27(1): 111-122.
2. Butte, M.J. et al., Mol. Immunol. 2008; 45: 3567-3572.
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