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Applications: Useful for studying protein binding and for screening small molecules and antibodies.
Description: Human Programmed Cell Death 1 Ligand 1 (PD-L1), also known as CD274 and B7 homolog 1 (B7-H1), PDCD1 ligand 1, programmed death ligand 1, PDCD1L1, GenBank Accession No. NM_014143, a.a. 19-239, with a C-terminal FLAG-tag, expressed in a HEK293 cell expression system. MW = 26 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_014143
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal FLAG-tag
Uniprot: Q9NZQ7
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al. J. Exp. Med. 2000; 192(7): 1027-1034
2. Dong, H., et al., Nat. Med. 1999; 5(12): 1365-1369.