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Encompassing Amino Acids: 19-239
Application: Useful for the study of protein-protein interaction, epitope mapping, and in vitro and in vivo protein function.
Description: Human secreted ProgrammedCell Death 1 Ligand 1 (PD-L1)-Fc fusionprotein, also known as CD274 and B7homolog 1 (B7-H1) with a R113A mutation,GenBank Accession No. NM_014143,amino acids 19-239, fused at the Cterminuswith the Fc portion of human IgG1,with C-terminal Avi-tag™ expressed in aHEK293 cell expression system andenzymatically biotin-labeled using Avi-tag™technology. MW = 54 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_014143
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
Uniprot: Q9NZQ7
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Freeman, G.J., et al. J. Exp. Med. 2000; 192(7): 1027-10342. Dong, H., et al., Nat. Med. 1999; 5(12): 1365-1369.
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