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Encompassing Amino Acids: 2-933(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE11A at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Mouse PDE11A (GenBank Accession No. NM_001081033), a.a. 2-933(end) with N-terminal GST tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_001081033
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal GST-tag
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fawcett, L. et al., PNAS. 97(7), 3207-3707 (2000).2. Horvath, A. et al., Nature Genetics. 38, 794-800 (2006).
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