Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 2-634(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE1C Assay Kit, Cat. # 60312. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl
2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1C at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE1C (GenBank Accession No. NM_005020), a.a. 2-634(end), with N-terminal GST-tag, MW=98 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04% Tween-20, and 20% glycerol
Genbank: NM_005020
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q14123
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Vandeput, F., et al. (2007). J. Biol. Chem. Nov 9;282(45):32749-57.
2. Cygnar, K.D., Zhao, H. (2009). Nat. Neurosci. Apr;12(4):454-62.
3. Ortiz-Capisano, M.C., et al. (2009). Am. J. Physiol. Regul. Integr. Comp. Physiol. Nov;297(5):R1469-76.Application Reference(s):
A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
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