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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's Mouse PDE1C Assay Kit, Cat. # 61312. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1C at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Mouse PDE1C (GenBank Accession No. NM_001025568), full length with N-terminal GST-tag, MW= 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 18 mM glutathione, 3 mM DTT, 10% glycerol
Genbank: NM_001025568
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q64338
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gong S. et al., Nature 425 (6961), 917-925 (2003).
2. Spence S. et al., Biochem. J. 321 (PT 1), 157- 163 (1997).
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