PDE2A, FLAG-tag Recombinant

PDE2A, FLAG-tag Recombinant
SKU
BPS60021
Packaging Unit
5 µg
Manufacturer
BPS Bioscience

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Encompassing Amino Acids: 2-941

Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 

Assay Conditions: 10 mM Tris-HCl, pH7.4, 10 mM MgCl2, 0.05% Tween-20,200 µM cAMP, and PDE2A1 at 37°C for20 min. Quantified by 5-nucleotidasecleaving the 5-AMP product andreleasing the phosphate group which isdetected by Malachite Green Reagent.

Description: Human PDE2A1 (GenBank Accession No. NM_002599), a.a. 2-941, with N-terminal FLAG-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Format: Aqueous buffer solution

Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 90 µg/ml FLAGpeptide, 10% glycerol, and 3 mM DTT.

Genbank: NM_002599

Host Cell Line: Sf9 cells

Storage Stability: At least 6 months at -80°C.

Tags: N-terminal FLAG-tag

Uniprot: O00408

Warnings: Avoid freeze/thaw cycles.

Biosafety Level: Not applicable (BSL-1)

References: 1. Seybold, J., et al., Blood 105 (9), 3569-3576 (2005).
2. Martinez, S.E., et al., Proc. Natl. Acad.Sci. U.S.A. 99 (20), 13260-13265(2002).
More Information
SKU BPS60021
Manufacturer BPS Bioscience
Manufacturer SKU 60021
Green Labware No
Package Unit 5 µg
Quantity Unit STK
Host Insect
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