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Encompassing Amino Acids: 343-998 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 0.02% TritonX, and serial dilutions of PDE3A at 37ºC for 20 min. Quantified by 5ʹ nucleotidase cleaving the 5ʹ-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Absorbance was measured at 620 nm.
Description: Dog PDE3A, also known as Phosphodiesterase 3A, CGMP-Inhibited 3',5'-Cyclic Phosphodiesterase A, and CGI-PDE A, GenBank Accession No. XP_005637137, a.a. 343-998 (end), with C-terminal FLAG-tag, expressed in a HEK293 expression system, MW = 75 KDa.
Format: Aqueous solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT, and 20% Glycerol.
Genbank: XP_005637137
Storage Stability: At least 6 months at -80°C.
Supplied As: Aqueous buffer solution.
Tags: FLAG-Tag
Uniprot: F1PL79
Warnings: Avoid multiple freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)
2. Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)
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