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Encompassing Amino Acids: 484-1141
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per min at 37°C. Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of Mouse PDE3A at 37°C for 20 min. Quantified by 5' nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Mouse PDE3A (GenBank Accession No. NM_018779), amino acids 484-1141 end), with N-terminal GST tag, MW = 100 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 18 mM Glutathione
Genbank: NM_018779
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: N-terminal GST-tag
Uniprot: B2RR84
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Hung, S.H., et al. J. Biol. Chem. 281 (39), 29236-29244 (2006)2.Hambleton, R., et al. J. Biol. Chem. 280 (47), 39168-39174 (2005)