Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 592-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE3B Assay Kit, Cat. # 60331. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl
2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE3B at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE3B (GenBank Accession No. NM_000922), a.a. 592-end, with N-terminal GST-tag, MW = 86 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris, pH 8.0, 110mM NaCl, 2.2 mM KCl, 3 mM DTT, 0.04%Tween-20, 20% glycerol
Genbank: NM_000922
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q14432
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Raymond, D.R., et al., Cell. Signal. 19 (12), 2507-2518 (2007).
2. Palmer, D., et al., J. Biol. Chem. 282 (13), 9411-9419 (2007).Application References:
1.
A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
2. Cyclic phosphatidic acid stimulates cAMP production and inhibits growth in human colon cancer cells (2013)
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