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Encompassing Amino Acids: 2-737(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Dog phosphodiesterase 4B (PDE4B) GenBank Accession No. XP_003434692, a.a. 2-737(end), with N-terminal GST-tag, MW 110 kDa, expressed in Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Genbank: XP_003434692
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: F1PJ12
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Girgenti, M.J., et al., PLoS One 2012,7(2):e32404.
2. Zhao, H., et al., Eur. J. Pharmacol. 2008 Aug 20,590(1-3):29-35.
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