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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 1 mM MnCl2, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4B1 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4B1 (GenBank Accession No. NM_001037341), full length with N-terminal GST-tag, MW= 109 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Genbank: NM_001037341
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q07343
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shah,V.O., et al., Am. J. Kidney Dis. 48 (4), 616-628 (2006)
2. Smith,P.G., et al., Blood 105 (1), 308-316 (2005).
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