Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: 121-end
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE7A kit, Cat. # 60370. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl
2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE7A at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Human PDE7A1 GenBank Accession No. NM_002603), a.a. 121-end with N-terminal GST-tag, MW=66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 4 mM Glutathione, 0.04% Tween-20, 3 mM DTT, and 20% glycerol.
Genbank: NM_002603
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q13946
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wang, H., et al., J. Biol. Chem. 280(35):30949-30955 (2005).
2. Torras-Llort, M. and Azorin, F., Biochem. J. 373(PT 3):835-843 (2003).Application References:
1.
Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
2. PDE7A1 hydrolyzes cCMP (2014)
The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
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