Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 108-446(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE7B kit, Cat. # 60371. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of mPDE7B at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Mouse PDE7B (GenBank Accession No. NM_013875), a.a. 108-446(end), with N-terminal GST-tag, MW=65 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mMglutathione, 10% glycerol, and 3 mM DTT.
Genbank: NM_013875
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9NP56
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Gardner, C., et al. (2000). Biochem Biophys Res Commun. May 27;272(1):186-92.
2. Reyes-Irisarri, E., et al. (2005). Neuroscience 132(4):1173-85.
3. Zhang, L., et al. (2008). Proc. Natl. Acad. Sci. USA Dec 9;105(49):19532-7.
English
Deutsch
