Products from BPS Bioscience require a minimum order value above 400€
Applications: Useful for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Description: Platelet-derived growth factor receptor A or PDGFRα has been implicated in regulation of cell growth and survival, apoptosis, and differentiation. It has been identified as a potential target in eosinophilic leukemia cancer, inflammatory breast cancer, and gastrointestinal stromal tumors. The D842 mutation is found in gastrointestinal stromal tumors and mutations at this site, other than D842Y, are associated with resistance to tyrosine kinase inhibitors. The PDGFRα (D842Y) Assay Kit is designed to measure PDGFRα (D842Y) activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent.
Storage Stability: Up to 6 months when stored as recommended.
Supplied As: The PDGFRα (D842Y) Assay Kit comes in a convenient 96-well format, with enough purified recombinant PDGFRα (D842Y), Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 96 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Evans, E., et al. A Precision Therapy Against Cancers Driven by KIT/PDGFRA Mutations; 2017, Science Translational Medicine Nov; 9(414): 1690.2. Joglekar-Javadekar, M., et al. Characterization and Targeting of Platelet-Derived Growth Factor Receptor alpha (PDGFRA) in Inflammatory Breast Cancer (IBC). Neoplasia. 2017 Jul; 19(7):564-573.3. Corless, C.L., et al. PDGFRA mutations in gastrointestinal stromal tumors: frequency, spectrum and in vitro sensitivity to imatinib. J Clin Oncol. 2005 Aug 10; 23(23):5357-64.
English
Deutsch
