Target: PDI
Conjugate: ATTO 390
Product Type: Polyclonal
Immunogen: Rat PDI synthetic peptide (409-509 aa C-terminal) conjugated to KLH
Swiss-Prot: P04785
Purification: Peptide Affinity Purified
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Concentration: 1 mg/ml
Specificity: Detects ~58kDa.
Cellular Localization: Endoplasmic Reticulum,Endoplasmic Reticulum Lumen
Scientific Background: The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation via its oxidase activity and isomerization via its isomerase activity, as well as the reduction of disulphide bonds in proteins (1). Studies suggest BiP and PDI work together sequentially to increase oxidation of these proteins (2, 3). PDI has also been found to function as a chaperone to prevent the aggregation of unfolded substrates, and serves as a subunit of prolyl 4-hydroxylase and microsomal triglyceride transferase (4, 5).PDI is an abundant 55kDa protein located primarily in the ER, however studies have also proved its presence in the cytosol (1). PDI has the ability to reside in the ER permanently due to the highly conserved KDEL sequence at its carboxy-terminus (6). It uses carboxy-terminal KDEL as a retention signal, and this appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor (7).
References: 1. NA K.S. et al. (2007) Mol Cells. 24(2): 261-7.2. Mayer M., Kies U., Kammermeier R., and Buchner J. (2000) J Biol Chem. 275(38): 29421-5.3. Delom F., Mallet B., Carayon P., and Lejeune P.J. (2001) J Biol Chem 276(24): 21337-42.4. Schultz-Norton J.R., McDonald W.H., Yates J.R. and Nardulli A.M. (2006) Mol Endocrinol 20(9): 1982-95.5. Turano C., Coppari S. Altieri F. and Ferraro (2002) J Cell Physiol 193: 154-163.6. Janiszewski M. (2005) J. Biol Chem. 280(49): 40813- 40819.7. Yoshimori T., et al. (1990) J Biol Chem. 265(26): 15984-90.
Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
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