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Encompassing Amino Acids: full length
Applications: Useful for the study of biochemical function, enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 3.45 µg of PRDX2 was incubated in 50 mM HEPES (pH 7.0) containing 200 µM NADPH, 3 µM thioredoxin, and 1.5 µM thioredoxin reductase. The reaction mixture was incubated at 30°C for 5 min, followed by the addition of 0.22 mM H2O2. NADPH oxidation was monitored (fluorescence decrease) for the next 10 min.
Description: Human Peroxiredoxin 2, also known as PRDX2, full length (Genbank accession number NM_005809) with N-terminal His-tag, MW=22.7 kDa, expressed in Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.35 mM KCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
Genbank: NM_005809
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P32119
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Peskin,A.V., et al., J. Biol. Chem. 282 (16), 11885-11892 (2007)
2. Low,F.M., et al., Blood 109 (6), 2611-2617 (2007)
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