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Encompassing Amino Acids: 2-520(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction was done in 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM β-D-fructose-6-phosphate, 100 µM ATP, 2 mM DTT, 5 mM KH2PO4, and various amount of PFKFB3 in a final volume of 50 µL. The reaction was incubated at 30°C for 30 min, and the amount of ATP consumed was calculated using Kinase-Glo® Plus reagent (Promega).
Description: Human 6-phosphofructo-2-kinase/ fructose-2,6-biphosphatase 3, also known as PFKFB3 and IPFK2, (GenBank Accession No. NM_004566), a.a. 2-520(end)) with N-terminal His-tag, MW = 60.4 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 360 mM imidazole and 20% glycerol
Genbank: NM_004566
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q16875
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cavalier, M.C., et al., Proteins 2012;80(4):1143-1153.
2. Reddy, M.M., et al., Leukemia 2012;26(3):481-489.
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