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Encompassing Amino Acids: 2-469
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: The reaction mixture contained 50 mM Tris-HCl, pH 7.5, 12.5 mM MgCl2, 1 mM -D-fructose-6- phosphate, 20 µM ATP, 2 mM DTT, 5 mM KH2PO4, and various amounts of PFKFB4 in a final volume of 50 uL. The reaction was incubated at 30°C for 30 min, the amount of ATP consumed was calculated using kinase-glo plus reagent (Promega).
Description: Human recombinant 6- phosphofructo-2-kinase/fructose-2,6- bisphosphatase 4 (PFKFB4), also known as 6PF-2-K/Fru-2,6-P2ase 4, and PFK/FBpase 4, a.a. 2-469 with an Nterminal His-tag, GenBank Accession No. NM_004567/NM_004558, MW = 55 kDa, expressed in an E. coli cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 200 mM imidazole and 20% glycerol.
Genbank: NM_004567/NP_004558
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His tag
Uniprot: Q16877
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1. Minchenko, O.H., et al., Acta Biochimica Poloncia. 2005; 52(4): 881-888.
2. Goidts, V., et al., Oncogene. 2012; 31: 3235-3243.
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