THUNDER Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT pan (S473) and Total AKT pan in cell lysates.

Components: Eu-phospho-AKTS473 (20 µL), Acc-phospho-AKTS473 (80 µL), Eu-Total AKT (5 µL), Acc-Total AKT (20 µL), AKT control lysate (500 µL), 5X Lysis Buffer 1 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-AKT pan (S473) & Total AKT pan.

Assay Target Class: Kinase.

SwissProt: P31749 , P31751 , Q9Y243.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-AKTS473PT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-AKTS473PT-500
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human, Mouse (Murine)
• Application: Cell-based Assay
• Human Gene ID: 207