THUNDER Phospho-JNK1/2/3 (T183/Y185) + Total JNK1/2/3 TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-JNK1/2/3 (T183/Y185) and Total JNK1/2/3 in cell lysates.

Components: Eu-phospho-JNK (20 µL), Acc-phospho-JNK (80 µL), Eu-Total JNK (5 µL), Acc-Total JNK (20 µL), JNK control lysate (500 µL), 5X Lysis Buffer 2 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-JNK1/2/3 (T183/Y185) and Total JNK1/2/3.

Assay Target Class: Kinase.

SwissProt: P45983, P45984 , P53779.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-JNKPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-JNKPT-500
• Green Labware: No
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 5599