THUNDER Phospho-NF-κB (S536) + Total NF-κB TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) and Total NF-κB in cell lysates.

Components: Eu-phospho-NFkB (20 µL), Acc-phospho-NFkB (80 µL), Eu-Total NFkB (5 µL), Acc-Total NFkB (20 µL), NFkB control lysate (500 µL), 5X Lysis Buffer 5 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-NF-κB (S536) and Total NF-κB.

Assay Target Class: Kinase.

SwissProt: Q04206.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-NFKBPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-NFKBPT-500
• Package Unit: 500 Points (400 point for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 5970