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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Complex of recombinant full-length human p110α (GenBank Accession No. NM_006218), with N-terminal His-Tag and recombinant full length, human p55γ (GenBank Accession No. NM_003629.2), no tag. Co-expressed in Sf9 cells in a Baculovirus expression system. p110α MW=111 kDa, p85α MW=61 kDa.
Format: Aqueous buffer solution
Formulation: 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Genbank: p110α: NM_006218, p55γ: NM_003629.2
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 - 0.5%).
Tags: p110α: N-terminal His-Tag, p55γ: No Tag
Uniprot: p110α: P42336; p55γ: Q8N381
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Terauchi, Y., et al. Nature Genet. 21: 230-235 (1999)2. Fukao, T., et al., Nature Immunol. 3: 295-304 (2002)
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