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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 25 µl kinase reaction is conducted in a buffer containing 40 mM Tris-HCl (pH 7.4), 20 mM MgCl2, 100 uM ATP, 200 µM PI:PS substrate (BPS catalog #40560) and 0-200 ng p120γ enzyme for 15 minutes at 30°C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform (Promega Corporation, Madison, WI).
Description: Human p120γ (gamma) (GenBank Accession No. NM_002649), full length with N-terminal His-tag, MW=130 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris, pH 8.0, 69 mM NaCl, 1.35 mM KCl, 0.025% Tween-20, 3 mM DTT, and 50% glycerol
Genbank: NM_002649
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P48736
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Webb, LM., et al., J Immunol. 175(5):2783-7 (2005).
2. Voigt, P., et al., J Biol Chem. 280(6):5121-7(2005).Application Reference:Discovery of a potent and isoform-selective targeted covalent inhibitor of the lipid kinase PI3Kα (2013)
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