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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Background: Protein kinase Cmu (PKCµ) is a member of the protein kinase C (PKC) family that differs from the other PKC isoenzymes in structural and enzymatic properties. PKCµ is a ubiquitous in nature with the highest expression in the thymus, lung and peripheral blood mononuclear cells (1). PKCµ forms a complex in vivo with a phosphatidylinositol 4-kinase and a phosphatidylinositol-4-phosphate 5-kinase. A region of PKCµ between the amino-terminal transmembrane domain and the pleckstrin homology domain is shown to be involved in the association with the lipid kinases (2).
Description: Recombinant human PKCµ (PKCmu), full length, with N-terminal GST-tag, expressed by Sf9 insect cells via a baculovirus expression system.
Format: Aqueous buffer solution
Formulation: 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol.
Genbank: X75756
Host Cell Line: Sf9 cells
Purity: ≥75%
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q15139
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rennecke, J. et al: Immunological demonstration of protein kinase C mu in murine tissues and various cell lines. Eur J Biochem. 1996 Dec 1;242(2):428-32.
2. Nishikawa, K. et al: Association of protein kinase Cmu with type II phosphatidylinositol 4-kinase and type phosphatidylinositol-4-phosphate 5-kinase. J Biol Chem. 1998 Sep 4;273(36):23126-33.
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