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Encompassing Amino Acids: 2-574 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 50 µl reaction is conducted in abuffer containing 50 mM Tris (pH 7.4), 10mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mMphosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo® LuminescentKinase Assay Platform.
Background: PKLR is a pyruvate kinase which catalyzes the transphosphorylation of phohsphoenolpyruvate into pyruvate and ATP. This reaction is the rate-limiting step of glycolysis. PKLR gene encodes the L- and R-type isoenzymes through alternate splicing events controlled by different promoters. L-type isoform can also appear as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young.
Description: Human pyruvate kinase variant 1 (PKR), GenBank Accession No. NM_000298, full length with N-terminal His-tag 2-574 (end), MW=63 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 3 mM DTT.
Genbank: NM_000298
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P30613
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Bullido M.J. et al., Neurobiol. Aging. 29(10): 1160-6 (2008).
2. Russell S.T. et al., Exp. Cell. Res. 315(1) : 16-25 (2009).
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