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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 50 µl reaction is conducted in abuffer containing 50 mM Tris (pH 7.4), 10mM MgCl2, 50 mM KCl, 2 mM ADP, 10 mMphosphoenolpyruvate (PEP) at roomtemperature for 15 min. ATP production isdetected using Kinase-Glo® LuminescentKinase Assay Platform.
Background: PKM1 and PKM2 are different splicing products of the PKM gene (exon 9 for PKM1 and exon 10 for PKM2). The M1 isoform (PKM1) is expressed in muscle and brain, and the M2 isoform is expressed during embryogenesis, in adipose tissue and pancreatic islets, and is the predominant form found in cancer cells.
Description: Human pyruvate kinase muscle (PKM1), GenBank Accession No. NM_182470, full length with N-terminal His-tag, MW=59 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 20% glycerol, and 3 mM DTT
Genbank: NM_182470
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: P14618
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Parkison C. et al., Biochem Biophys Res Commun. 179(1) ; 668-74 (1991).
2. Bluemlein K. et al., Oncotarget. 2011 May 22.
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