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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35 and 2 µM substrate (Ser/Thr Peptide 16, Invitrogen) at 30° for 1 hour.
Background: PLK1 is a member of the Polo-Like Kinase family that localize to centrosomes or spindle pole bodies and undergo dramatic subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in abnormalities in centrosome duplication, maturation, and/or microtubule dynamics. PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies, and ectopic expression of PLK1 dominant negative protein leads to rapid cell death.
Description: Human Plk1 (GenBank Accession No. NM_005030), full length with C-terminal His-tag, MW=68 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT and 10% glycerol
Genbank: NM_005030
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: P53350
Unit Definition: One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of substrate per minute at pH 7.5 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Fabbro, M. et al., Dev. Cell 9 (4), 477-488. (2005).
2. Liu, X., et al., Mol. Cell. Biol. 25, 4993-5010 (2005).
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